Scholarlink

<p>The esterase gene encoding EstJN1 of <i>Clostridium butyricum</i>, which was isolated from the pit cellar of Chinese liquor facility, was expressed. EstJN1 was identified as a novel GDSL esterase belonging to family II. The enzyme demonstrated a marked substrate preference for <i>p</i>-nitrophenyl butyrate, with optimal activity at a temperature of 40 ℃ and a pH of 7.0. Notably, EstJN1 displayed prominent tolerance to acidic conditions and organic solvents, particularly methanol, ethanol, and ethyl acetate. Moreover, the whole-cell biocatalyst of EstJN1 was found to efficiently catalyze the synthesis of ethyl hexanoate in heptane, achieving ester conversion rate of 80.5% under the optimized conditions of 40 ℃ temperature, 0.25&#xa0;M ethanol and 0.2&#xa0;M hexanoic acid concentrations. The distinctive characteristics of EstJN1 suggest a potential role in the biosynthesis of flavor compounds in Chinese liquor. EstJN1 has promising application in the synthesis of flavor esters and enhancement of aromatic profiles of alcoholic beverages.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Expression and characterization of a Clostridium Butyricum esterase from pit cellar of Chinese liquor and its potential in flavor ester synthesis

  • 期刊论文

  • 发表日期：

    2025年9月8日

查看全文
  • Chuan Wang,
  • Ge Rang Lamu,
  • Xiaomei Wang,
  • Xueping Wen,
  • Li Li

摘要

The esterase gene encoding EstJN1 of Clostridium butyricum, which was isolated from the pit cellar of Chinese liquor facility, was expressed. EstJN1 was identified as a novel GDSL esterase belonging to family II. The enzyme demonstrated a marked substrate preference for p-nitrophenyl butyrate, with optimal activity at a temperature of 40 ℃ and a pH of 7.0. Notably, EstJN1 displayed prominent tolerance to acidic conditions and organic solvents, particularly methanol, ethanol, and ethyl acetate. Moreover, the whole-cell biocatalyst of EstJN1 was found to efficiently catalyze the synthesis of ethyl hexanoate in heptane, achieving ester conversion rate of 80.5% under the optimized conditions of 40 ℃ temperature, 0.25 M ethanol and 0.2 M hexanoic acid concentrations. The distinctive characteristics of EstJN1 suggest a potential role in the biosynthesis of flavor compounds in Chinese liquor. EstJN1 has promising application in the synthesis of flavor esters and enhancement of aromatic profiles of alcoholic beverages.