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<p>A <i>Lactobacillus</i>-rich vaginal microbiome is associated with a reduced risk for sexually transmitted diseases and adverse reproductive health outcomes, with <i>Lactobacillus crispatus</i> identified as particularly beneficial. This study investigated the nutritional requirements of two vaginal isolates, <i>L. crispatus</i> RL09 and RL10, and presents a chemically defined medium (CDM) that supports their growth. This study experimentally validated that <i>L. crispatus</i> relies on exogenous fatty acids, essential B vitamins, including riboflavin (B2), niacin (B3) and pantothenate (B5), and all eighteen amino acids for sustained growth. On one hand, this body of work adds to the physiological understanding of <i>Lactobacilliaceae</i> and lays the groundwork for future quantitative studies on <i>L. crispatus</i>. On the other hand, we have shown that <i>L. crispatus</i> exhibits a high metabolic dependency on its environment. These dependencies underscore the potential sensitivity of <i>L. crispatus</i> to nutrient variations, which may influence its ability to dominate and maintain a healthy vaginal ecosystem.</p>

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Insights into host dependency from a chemically defined medium for the human vaginal bacterium Lactobacillus crispatus

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月6日

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  • P. Achterberg,
  • N. J. K. van Geenen,
  • R. Y. Hertzberger,
  • D. Molenaar,
  • M. C. M. van Loosdrecht,
  • R. Kort

摘要

A Lactobacillus-rich vaginal microbiome is associated with a reduced risk for sexually transmitted diseases and adverse reproductive health outcomes, with Lactobacillus crispatus identified as particularly beneficial. This study investigated the nutritional requirements of two vaginal isolates, L. crispatus RL09 and RL10, and presents a chemically defined medium (CDM) that supports their growth. This study experimentally validated that L. crispatus relies on exogenous fatty acids, essential B vitamins, including riboflavin (B2), niacin (B3) and pantothenate (B5), and all eighteen amino acids for sustained growth. On one hand, this body of work adds to the physiological understanding of Lactobacilliaceae and lays the groundwork for future quantitative studies on L. crispatus. On the other hand, we have shown that L. crispatus exhibits a high metabolic dependency on its environment. These dependencies underscore the potential sensitivity of L. crispatus to nutrient variations, which may influence its ability to dominate and maintain a healthy vaginal ecosystem.