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<p>This study investigated the impacts of penicillin G on the <i>Chlamydia trachomatis</i> (CT) Serovar D and host cells in urogenital infections. HeLa cells were infected with CT for 2&#xa0;h and then treated with different concentrations of penicillin G. The aberrant CT group was compared to untreated CT using dual RNA sequencing, lncRNA sequencing, and bioinformatics analysis. Treatment with 150 U/ml penicillin G for 48&#xa0;h caused significant abnormalities in CT inclusion bodies. A total of 93 differentially expressed mRNAs were involved in biological processes such as protein modification, cofactor biosynthesis, and response to stimuli. Key pathways affected included peptidoglycan biosynthesis and degradation, oxidative phosphorylation, and bacterial secretion systems. Additionally, penicillin G induced significant changes in CT morphology and mRNA expression, as well as in host HeLa cellular mRNA and lncRNA profiles. These changes may contribute to chronic inflammation, treatment resistance, and potentially tumorigenesis.</p>

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Impacts of penicillin G on Chlamydia trachomatis serovar D and host HeLa cells in genital infections

  • 期刊论文

  • 发表日期：

    2025年5月31日

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  • Mei Wang,
  • Xiufeng Qiu,
  • Youhong Xu,
  • Leran Zhao,
  • Quanzhong Liu,
  • Cong You

摘要

This study investigated the impacts of penicillin G on the Chlamydia trachomatis (CT) Serovar D and host cells in urogenital infections. HeLa cells were infected with CT for 2 h and then treated with different concentrations of penicillin G. The aberrant CT group was compared to untreated CT using dual RNA sequencing, lncRNA sequencing, and bioinformatics analysis. Treatment with 150 U/ml penicillin G for 48 h caused significant abnormalities in CT inclusion bodies. A total of 93 differentially expressed mRNAs were involved in biological processes such as protein modification, cofactor biosynthesis, and response to stimuli. Key pathways affected included peptidoglycan biosynthesis and degradation, oxidative phosphorylation, and bacterial secretion systems. Additionally, penicillin G induced significant changes in CT morphology and mRNA expression, as well as in host HeLa cellular mRNA and lncRNA profiles. These changes may contribute to chronic inflammation, treatment resistance, and potentially tumorigenesis.