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<p>Within a citizen science project, 112 freshwater habitats in Austria were sampled to get bacterial cultures belonging to the genus <i>Aquirufa</i> using a strategy for targeted isolation. We focused on these bacteria because they are widespread and represent typical freshwater bacteria and, furthermore, the typic red pigmentation facilitates preselection. Among the 113 obtained <i>Aquirufa</i> strains were HETE-83D<sup>T</sup>, KTFRIE-69F<sup>T</sup>, OSTEICH-129V<sup>T</sup> and PLAD-142S6K<sup>T</sup>, originating from small ponds and a creek. Phylogenetic reconstructions with 16S rRNA gene sequences and genome-based analyses with amino acid sequences of 501 core genes showed that all four strains belonged to the <i>A. antheringensis</i> branch of the genus <i>Aquirufa</i>. Calculation of whole-genome average nucleotide identity values and digital DNA-DNA hybridization values revealed that they represent in each case a new species. The genome sizes of the four strains were between 2.5 and 2.8 Mbp and the G + C values were between 41.4 and 41.8%. Like all type strains of the genus <i>Aquirufa</i>, cells were rod-shaped, and liquid cultures and colonies on agar plates were red-pigmented, likely due to carotenoids. All strains except OSTEICH-129V<sup>T</sup> showed gliding motility on soft agar plates. All strains grew aerobically but only PLAD-142S6K<sup>T</sup> could grow weakly under anaerobic conditions. We propose here to establish the names <i>Aquirufa esocilacus</i> sp. nov. for strain HETE-83D<sup>T</sup> (= DSM 118087<sup>T</sup> = JCM 37094<sup>T</sup>), <i>Aquirufa originis</i> sp. nov. for KTFRIE-69F<sup>T</sup> (= DSM 117798<sup>T</sup> = JCM 37095<sup>T</sup>), <i>Aquirufa avitistagni</i> for OSTEICH-129V<sup>T</sup> (= DSM 118088<sup>T</sup> = JCM 37100<sup>T</sup>) and <i>Aquirufa echingensis</i> sp. nov. for PLAD-142S6K<sup>T</sup> (= DSM 117799<sup>T</sup> = JCM 37096<sup>T</sup>).</p>

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Aquirufa esocilacus sp. nov., Aquirufa originis sp. nov., Aquirufa avitistagni, and Aquirufa echingensis sp. nov. discovered in small freshwater habitats in Austria during a citizen science project

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  • 发表日期：

    2025年2月25日

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  • Alexandra Pitt,
  • Stefan Lienbacher,
  • Johanna Schmidt,
  • Meina Neumann-Schaal,
  • Jacqueline Wolf,
  • Aharon Oren,
  • Sophia Reichl,
  • Martin W. Hahn

摘要

Within a citizen science project, 112 freshwater habitats in Austria were sampled to get bacterial cultures belonging to the genus Aquirufa using a strategy for targeted isolation. We focused on these bacteria because they are widespread and represent typical freshwater bacteria and, furthermore, the typic red pigmentation facilitates preselection. Among the 113 obtained Aquirufa strains were HETE-83DT, KTFRIE-69FT, OSTEICH-129VT and PLAD-142S6KT, originating from small ponds and a creek. Phylogenetic reconstructions with 16S rRNA gene sequences and genome-based analyses with amino acid sequences of 501 core genes showed that all four strains belonged to the A. antheringensis branch of the genus Aquirufa. Calculation of whole-genome average nucleotide identity values and digital DNA-DNA hybridization values revealed that they represent in each case a new species. The genome sizes of the four strains were between 2.5 and 2.8 Mbp and the G + C values were between 41.4 and 41.8%. Like all type strains of the genus Aquirufa, cells were rod-shaped, and liquid cultures and colonies on agar plates were red-pigmented, likely due to carotenoids. All strains except OSTEICH-129VT showed gliding motility on soft agar plates. All strains grew aerobically but only PLAD-142S6KT could grow weakly under anaerobic conditions. We propose here to establish the names Aquirufa esocilacus sp. nov. for strain HETE-83DT (= DSM 118087T = JCM 37094T), Aquirufa originis sp. nov. for KTFRIE-69FT (= DSM 117798T = JCM 37095T), Aquirufa avitistagni for OSTEICH-129VT (= DSM 118088T = JCM 37100T) and Aquirufa echingensis sp. nov. for PLAD-142S6KT (= DSM 117799T = JCM 37096T).