Introduction and Hypothesis <p>This study investigates the postoperative mobility following partial semitendinosus tendon harvesting performed during pelvic organ prolapse (POP) repair using the Hornemann Tendon Transplantation (HoTT®) method. The aim was to evaluate whether the procedure causes functional impairments and which patient-related factors influence postoperative mobility outcomes.</p> Methods <p>A single-arm prospective observational study was conducted involving 142 women with POP-Q stage II–IV prolapse who underwent laparoscopic sacropexy with partial autologous semitendinosus tendon harvest. Mobility was assessed preoperatively and at 6&#xa0;weeks, 3&#xa0;months, and 6&#xa0;months postoperatively using the validated Prosthetic Limb Users Survey of Mobility (PLUS-M™). Statistical analysis included mixed logistic regression with age, BMI, and time as fixed effects and patient identity as a random effect.</p> Results <p>Mean age was 60.1 ± 10.3&#xa0;years; mean BMI was 24.3 ± 3.7&#xa0;kg/m². Median mobility (<i>T</i>-score) increased from 64.5 before surgery to 67.1 at 6&#xa0;weeks and 71.4 at both 3 and 6&#xa0;months (<i>p</i> &lt; 0.001). Higher age (OR 0.94; <i>p</i> = 0.022) and BMI (OR 0.82; <i>p</i> = 0.009) were independently associated with lower odds of achieving a high mobility score (<i>T</i> ≥ 71.4). No significant change in continence status was observed. Patient satisfaction and quality of life improved markedly, with 96.6% recommending the procedure.</p> Conclusions <p>Partial semitendinosus tendon harvesting in HoTT® surgery does not impair mobility and is associated with significant improvements in function and quality of life. Advanced age and higher BMI mildly reduce postoperative mobility potential but do not contraindicate the procedure.</p>