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<p>The complexity of potato genetics, characterised by tetrasomic inheritance, has contributed to slower genetic gain in potato compared to other major crops. Disease resistance genes, often found in large clusters of highly similar paralogs and alleles, further complicate genetic studies. The <i>H1</i> resistance locus, introgressed into potato cultivars from <i>Solanum tuberosum</i> ssp. <i>andigena</i>, has been successfully used for over 60&#xa0;years to control <i>Globodera rostochiensis</i> in Europe. Although previous genetic studies mapped this resistance to chromosome 5, the complete structure of the locus remained elusive. To reduce genomic complexity, we generated a dihaploid of the cultivar ‘Athlete’, DH4_Athlete, carrying the <i>H1</i> resistance locus, and produced a phased haplotype representation of the <i>H1</i> interval using Oxford Nanopore sequencing. Combined with RenSeq-based association genetics, this approach allowed us to reconstruct the entire <i>H1</i> locus, including recombination points at both the 5′ and 3′ ends of the interval.</p>

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Phased potato genome assembly and association genetics enable delineation of the H1 resistance locus against potato cyst nematodes

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月12日

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  • Yuk Woon Cheung,
  • Lynn H. Brown,
  • Thomas M. Adams,
  • Brian Harrower,
  • Amanpreet Kaur,
  • Gaynor McKenzie,
  • Jamie Orr,
  • James Price,
  • Vikrant Singh,
  • Moray Smith,
  • Micha Bayer,
  • Ingo Hein

摘要

The complexity of potato genetics, characterised by tetrasomic inheritance, has contributed to slower genetic gain in potato compared to other major crops. Disease resistance genes, often found in large clusters of highly similar paralogs and alleles, further complicate genetic studies. The H1 resistance locus, introgressed into potato cultivars from Solanum tuberosum ssp. andigena, has been successfully used for over 60 years to control Globodera rostochiensis in Europe. Although previous genetic studies mapped this resistance to chromosome 5, the complete structure of the locus remained elusive. To reduce genomic complexity, we generated a dihaploid of the cultivar ‘Athlete’, DH4_Athlete, carrying the H1 resistance locus, and produced a phased haplotype representation of the H1 interval using Oxford Nanopore sequencing. Combined with RenSeq-based association genetics, this approach allowed us to reconstruct the entire H1 locus, including recombination points at both the 5′ and 3′ ends of the interval.