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<p>Message.</p><p>A QTL from rye chromosome 5R confers resistance to root-lesion nematode in triticale.</p><p>Root-lesion nematode (<i>Pratylenchus neglectus</i>, RLN) poses a significant threat to global wheat production. High levels of RLN resistance are rare in wheat. Triticale, an amphiploid generated by combining wheat and rye genomes that naturally carries rye-derived defense alleles, offers an untapped reservoir of nematode resistance. Here, we evaluated the response to RLN in 137 recombinant inbred lines (RILs) derived from a cross between two triticale cultivars: Siskiyou (susceptible) and Villax St. Jose (resistant). Genotyping-by-sequencing identified 1054 high-quality single-nucleotide polymorphism (SNP) markers, which, along with seven simple sequence repeat (SSR) markers, were assembled into 21 linkage groups covering the triticale genome. A single quantitative trait locus (QTL) on the rye-derived chromosome 5R was identified that explained approximately 20% of the phenotypic variance across experiments. A high-throughput Kompetitive allele-specific PCR (KASP) assay based on the most significant SNP marker was developed, providing a rapid genotyping platform for selecting the resistance allele and reducing reliance on labor-intensive phenotyping for <i>P. neglectus</i> resistance in triticale. This study reports the first mapped RLN-resistance QTL in triticale, laying the fundamental foundation for introgressing the 5R resistance allele into wheat via marker-assisted selection combined with chromosome engineering, thereby broadening the genetic basis for nematode resistance in cereal crops.</p>

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Genetic analysis of a quantitative trait locus associated with resistance to the root-lesion nematode Pratylenchus neglectus in triticale

  • 期刊论文

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  • 发表日期：

    2026年1月5日

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  • Gurminder Singh,
  • Krishna Acharya,
  • Bonventure Mumia,
  • Siddant Ranabhat,
  • Ekta Ojha,
  • Jatinder Singh,
  • Upinder Gill,
  • Sean Walkowiak,
  • Harmeet Singh Chawla,
  • Xuehui Li,
  • Justin Faris,
  • Zhaohui Liu,
  • Guiping Yan

摘要

Message.

A QTL from rye chromosome 5R confers resistance to root-lesion nematode in triticale.

Root-lesion nematode (Pratylenchus neglectus, RLN) poses a significant threat to global wheat production. High levels of RLN resistance are rare in wheat. Triticale, an amphiploid generated by combining wheat and rye genomes that naturally carries rye-derived defense alleles, offers an untapped reservoir of nematode resistance. Here, we evaluated the response to RLN in 137 recombinant inbred lines (RILs) derived from a cross between two triticale cultivars: Siskiyou (susceptible) and Villax St. Jose (resistant). Genotyping-by-sequencing identified 1054 high-quality single-nucleotide polymorphism (SNP) markers, which, along with seven simple sequence repeat (SSR) markers, were assembled into 21 linkage groups covering the triticale genome. A single quantitative trait locus (QTL) on the rye-derived chromosome 5R was identified that explained approximately 20% of the phenotypic variance across experiments. A high-throughput Kompetitive allele-specific PCR (KASP) assay based on the most significant SNP marker was developed, providing a rapid genotyping platform for selecting the resistance allele and reducing reliance on labor-intensive phenotyping for P. neglectus resistance in triticale. This study reports the first mapped RLN-resistance QTL in triticale, laying the fundamental foundation for introgressing the 5R resistance allele into wheat via marker-assisted selection combined with chromosome engineering, thereby broadening the genetic basis for nematode resistance in cereal crops.