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Key message <p>The <i>CsaV3_1G006020</i> (<i>CsAFP3-like</i>) gene, which controlled determinate growth of lateral branch in cucumber via the ABA signaling pathway, was identified.</p> Abstract <p>Lateral branching is a critical agronomic trait in cucumber that directly influences plant architecture and crop yield. Therefore, understanding the genetic mechanisms underlying this trait is essential for effective cucumber breeding. Here, we identified a branchless mutant, designated <i>mbl</i> (<i>mutant-branchless</i>), exhibiting extremely determinate growth of lateral branch. Using BSA-seq analysis with four DNA bulk pools derived from an <i>F</i><sub>2</sub> population crossing between <i>mbl</i> and a normal lateral branching variety, EC1, we mapped distinct loci associated with the lateral branch development. The <i>A-Det</i> (<i>Main axis-determinate growth</i>) was localized to chromosome 6 and corresponded to the previously reported <i>CsTFL1</i> gene. The<i> B-NDI</i> (<i>Lateral branch-node differentiation inhibiting</i>) locus was located on chromosome 3, with the candidate region narrowed down to 3.03 Mb. The <i>B-det</i> (<i>Lateral branch-determinate growth</i>) was fine-mapped to a 391.74&#xa0;kb interval on chromosome 1, within which only one non-synonymous mutation was found in <i>CsaV3_1G006020</i> (<i>CsAFP3-like</i>). CsAFP3-like encoded an ABA INSENSITIVE 5 (ABI5) binding protein, of which the mutation located in the conserved ABI5 binding domain. <i>CsAFP3-like</i> expression was significantly decreased in leaf axils of <i>mbl</i> compared to EC1, where the endogenous ABA was dramatically accumulated. The&#xa0;<i>CsAFP3-like</i> VIGS experiment in cucumber confirmed its role in regulating determinate growth of lateral branch. These findings suggest that <i>CsAFP3-like</i> positively regulates ABA-mediated inhibition of lateral branch development.</p>

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Identification of CsAFP3-like as a novel gene regulating determinate growth of lateral branch in cucumber

  • 期刊论文

  • 发表日期：

    2025年11月18日

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  • Fan Yang,
  • Yan Xu,
  • Zichun Wang,
  • Biao Dong,
  • Siwen Zhou,
  • Minying Jiang,
  • Ji Li

摘要

Key message

The CsaV3_1G006020 (CsAFP3-like) gene, which controlled determinate growth of lateral branch in cucumber via the ABA signaling pathway, was identified.

Abstract

Lateral branching is a critical agronomic trait in cucumber that directly influences plant architecture and crop yield. Therefore, understanding the genetic mechanisms underlying this trait is essential for effective cucumber breeding. Here, we identified a branchless mutant, designated mbl (mutant-branchless), exhibiting extremely determinate growth of lateral branch. Using BSA-seq analysis with four DNA bulk pools derived from an F2 population crossing between mbl and a normal lateral branching variety, EC1, we mapped distinct loci associated with the lateral branch development. The A-Det (Main axis-determinate growth) was localized to chromosome 6 and corresponded to the previously reported CsTFL1 gene. The B-NDI (Lateral branch-node differentiation inhibiting) locus was located on chromosome 3, with the candidate region narrowed down to 3.03 Mb. The B-det (Lateral branch-determinate growth) was fine-mapped to a 391.74 kb interval on chromosome 1, within which only one non-synonymous mutation was found in CsaV3_1G006020 (CsAFP3-like). CsAFP3-like encoded an ABA INSENSITIVE 5 (ABI5) binding protein, of which the mutation located in the conserved ABI5 binding domain. CsAFP3-like expression was significantly decreased in leaf axils of mbl compared to EC1, where the endogenous ABA was dramatically accumulated. The CsAFP3-like VIGS experiment in cucumber confirmed its role in regulating determinate growth of lateral branch. These findings suggest that CsAFP3-like positively regulates ABA-mediated inhibition of lateral branch development.