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Key message <p>The glutathione S-transferase family gene <i>OsGSTT3</i> in rice regulates seed germination by modulating reactive oxygen species (ROS) homeostasis. The relative expression levels of ROS scavenging-related genes were changed.</p> Abstract <p>Seed germination is a complex physiological process regulated by both internal and external factors. Glutathione S-transferases (GSTs), a critical class of antioxidant enzymes, play key roles in plant responses to environmental stress. However, their molecular regulatory mechanisms in rice seed germination remain largely unexplored. In this study, we identified <i>OsGSTT3</i> as a key regulator of seed germination in rice. Both <i>OsGSTT3</i> overexpression and knockout lines exhibited delayed germination speeds compared with wild-type (WT) plants. RT-qPCR analysis revealed that <i>OsGSTT3</i> is highly expressed in seeds and during germination, with its expression modulated by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and diphenyleneiodonium chloride (DPI). Exogenous H<sub>2</sub>O<sub>2</sub> and DPI treatments further confirmed that reactive oxygen species (ROS) levels are critical determinants of germination in <i>OsGSTT3</i> transgenic lines. Measurements of endogenous H<sub>2</sub>O<sub>2</sub> revealed significantly reduced levels in overexpression lines and increased levels in knockout lines relative to WT. Additionally, <i>OsGSTT3</i> regulates ROS homeostasis during germination by modulating the expression of ROS scavenging-related genes. Collectively, our findings establish <i>OsGSTT3</i> as a key regulator of seed germination via ROS homeostasis, providing novel insights into the molecular mechanisms of rice germination and offering a potential genetic resource for improving rice germplasm.</p>

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OsGSTT3 regulates seed germination by modulating reactive oxygen species homeostasis in rice

  • 期刊论文

  • 发表日期：

    2025年11月11日

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  • Yunyi Wen,
  • Yanjin Zhou,
  • Mingyang Ding,
  • Zilong Luo,
  • Can Wang,
  • Yibin Pan,
  • Ying He,
  • Dagang Jiang

摘要

Key message

The glutathione S-transferase family gene OsGSTT3 in rice regulates seed germination by modulating reactive oxygen species (ROS) homeostasis. The relative expression levels of ROS scavenging-related genes were changed.

Abstract

Seed germination is a complex physiological process regulated by both internal and external factors. Glutathione S-transferases (GSTs), a critical class of antioxidant enzymes, play key roles in plant responses to environmental stress. However, their molecular regulatory mechanisms in rice seed germination remain largely unexplored. In this study, we identified OsGSTT3 as a key regulator of seed germination in rice. Both OsGSTT3 overexpression and knockout lines exhibited delayed germination speeds compared with wild-type (WT) plants. RT-qPCR analysis revealed that OsGSTT3 is highly expressed in seeds and during germination, with its expression modulated by hydrogen peroxide (H2O2) and diphenyleneiodonium chloride (DPI). Exogenous H2O2 and DPI treatments further confirmed that reactive oxygen species (ROS) levels are critical determinants of germination in OsGSTT3 transgenic lines. Measurements of endogenous H2O2 revealed significantly reduced levels in overexpression lines and increased levels in knockout lines relative to WT. Additionally, OsGSTT3 regulates ROS homeostasis during germination by modulating the expression of ROS scavenging-related genes. Collectively, our findings establish OsGSTT3 as a key regulator of seed germination via ROS homeostasis, providing novel insights into the molecular mechanisms of rice germination and offering a potential genetic resource for improving rice germplasm.