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<p>Stripe rust (<i>Puccinia striiformis</i> f. sp. <i>tritici</i>) is a globally devastating foliar disease affecting common wheat. The development of new wheat varieties with novel and durable stripe rust resistance through wide hybridization represents a sustainable and cost-effective strategy for controlling the disease. <i>Thinopyrum ponticum</i> (Podp.) Barkworth &amp; D.R. Dewey, a polyploid species in the tertiary genetic pool of wheat, harbors multiple disease resistance genes, and has been widely utilized for wheat improvement. We previously identified a wheat-<i>Th. ponticum</i> 6J<sup>S</sup> (6B) substitution line X005 with novel stripe rust resistance, which was derived from the wheat-<i>Th. ponticum</i> partial amphiploid Xiaoyan7430. In the present study, the chromosome compositions of Xiaoyan7430 and X005 were precisely characterized using non-denaturing&#xa0;-&#xa0;fluorescence <i>in situ</i> hybridization (ND-FISH) and Oligo-FISH painting. Notably, chromosome 6J<sup>S</sup> in X005 displayed distinctly different ND-FISH patterns compared to other reported <i>Th. ponticum</i>-derived 6Ae chromosomes introduced into various wheat backgrounds. To physically localize the 6J<sup>S</sup>-derived stripe rust resistance gene from X005, we isolated a set of wheat-6J<sup>S</sup> deletion and translocation lines after extensive screening of the progenies of crosses between X005 and susceptible wheat cultivars. Using 75 molecular markers, we established a cytological bin map for chromosome 6J<sup>S</sup> of X005. Resistance evaluation combined with molecular mapping revealed that the critical resistance locus resides in bin 6J<sup>S</sup>S-2 (FL&#xa0;0.53–0.67) on the 6J<sup>S</sup> short arm, corresponding to the 74.51–135.61&#xa0;Mb genome region of <i>Th. elongatum</i> chromosome 6E. This locus confers stripe rust resistance at both the seedling and adult-plant stages. Translocation T6J<sup>S</sup>S·6BL had enhanced <i>Yr</i> resistance and increased tiller numbers without any obvious negative effect on agronomic traits. Thus, the newly developed wheat-<i>Th. ponticum</i> 6J<sup>S</sup> translocation lines represent novel germplasm resources for the breeding of disease-resistant wheat cultivars.</p>

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Molecular characterization of a new wheat-Thinopyrum ponticum translocation line with resistance to stripe rust

  • 期刊论文

  • 发表日期：

    2025年8月22日

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  • Chengzhi Jiang,
  • Yujie Luo,
  • Yile Qi,
  • Li Li,
  • Tingting Jiang,
  • Ennian Yang,
  • Guangrong Li,
  • Zujun Yang

摘要

Stripe rust (Puccinia striiformis f. sp. tritici) is a globally devastating foliar disease affecting common wheat. The development of new wheat varieties with novel and durable stripe rust resistance through wide hybridization represents a sustainable and cost-effective strategy for controlling the disease. Thinopyrum ponticum (Podp.) Barkworth & D.R. Dewey, a polyploid species in the tertiary genetic pool of wheat, harbors multiple disease resistance genes, and has been widely utilized for wheat improvement. We previously identified a wheat-Th. ponticum 6JS (6B) substitution line X005 with novel stripe rust resistance, which was derived from the wheat-Th. ponticum partial amphiploid Xiaoyan7430. In the present study, the chromosome compositions of Xiaoyan7430 and X005 were precisely characterized using non-denaturing - fluorescence in situ hybridization (ND-FISH) and Oligo-FISH painting. Notably, chromosome 6JS in X005 displayed distinctly different ND-FISH patterns compared to other reported Th. ponticum-derived 6Ae chromosomes introduced into various wheat backgrounds. To physically localize the 6JS-derived stripe rust resistance gene from X005, we isolated a set of wheat-6JS deletion and translocation lines after extensive screening of the progenies of crosses between X005 and susceptible wheat cultivars. Using 75 molecular markers, we established a cytological bin map for chromosome 6JS of X005. Resistance evaluation combined with molecular mapping revealed that the critical resistance locus resides in bin 6JSS-2 (FL 0.53–0.67) on the 6JS short arm, corresponding to the 74.51–135.61 Mb genome region of Th. elongatum chromosome 6E. This locus confers stripe rust resistance at both the seedling and adult-plant stages. Translocation T6JSS·6BL had enhanced Yr resistance and increased tiller numbers without any obvious negative effect on agronomic traits. Thus, the newly developed wheat-Th. ponticum 6JS translocation lines represent novel germplasm resources for the breeding of disease-resistant wheat cultivars.