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Key Message <p> It was hypothesized that <i>Bch08G003160</i> (<i>CqMYB62</i>), located in the 51.08&#xa0;Kb region on chromosome 08, might be an important candidate gene for the subgynoecy trait in chieh-qua, based on BSA-seq and linkage mapping approaches.</p> Abstract <p> In cucurbit crops, the use of female lines can greatly increase the yield of a single plant and is especially important for the production of hybrid generation seeds, thus being of great interest to breeders. To identify genes regulating sex differentiation in chieh-qua, genetic analysis of the subgynoecy trait was conducted using a chieh-qua F<sub>2</sub> population. Initial localization of the locus was done using BSA-seq, followed by fine mapping with a large <i>F</i><sub>2</sub> population (<i>n</i> = 2,741). The locus was ultimately narrowed down to a 51.08&#xa0;Kb region on Chr08, revealing a single gene <i>Bch08G003160</i> (<i>CqMYB62</i>) in this region. Further analysis revealed that the presence of two variant loci (SNP_416 and SNP_317) in the coding region resulted in premature termination of the codon and loss of function of the structural domain of the PLN03212 superfamily. Moreover, our research indicated that the subgynoecy trait mediated by <i>CqMYB62</i> in chieh-qua is potentially regulated by gibberellic acid (GA). Two efficient dCAPS markers were developed to distinguish subgynoecy. In summary, these findings highlight the critical role of <i>CqMYB62</i> in subgynoecy trait regulation, offering potential implications for chieh-qua breeding programs.</p>

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Fine mapping and identification of CqMYB62 as the subgynoecy gene in chieh-qua (Benincasa hispida Cogn. var. Chieh-qua How)

  • 期刊论文

  • 发表日期：

    2025年4月9日

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  • Yin Gao,
  • Jiazhu Peng,
  • Yanchun Qiao,
  • Guoping Wang,
  • Jianpo Zhan,
  • Wensheng Zhang

摘要

Key Message

It was hypothesized that Bch08G003160 (CqMYB62), located in the 51.08 Kb region on chromosome 08, might be an important candidate gene for the subgynoecy trait in chieh-qua, based on BSA-seq and linkage mapping approaches.

Abstract

In cucurbit crops, the use of female lines can greatly increase the yield of a single plant and is especially important for the production of hybrid generation seeds, thus being of great interest to breeders. To identify genes regulating sex differentiation in chieh-qua, genetic analysis of the subgynoecy trait was conducted using a chieh-qua F2 population. Initial localization of the locus was done using BSA-seq, followed by fine mapping with a large F2 population (n = 2,741). The locus was ultimately narrowed down to a 51.08 Kb region on Chr08, revealing a single gene Bch08G003160 (CqMYB62) in this region. Further analysis revealed that the presence of two variant loci (SNP_416 and SNP_317) in the coding region resulted in premature termination of the codon and loss of function of the structural domain of the PLN03212 superfamily. Moreover, our research indicated that the subgynoecy trait mediated by CqMYB62 in chieh-qua is potentially regulated by gibberellic acid (GA). Two efficient dCAPS markers were developed to distinguish subgynoecy. In summary, these findings highlight the critical role of CqMYB62 in subgynoecy trait regulation, offering potential implications for chieh-qua breeding programs.