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<p>Amphibians, as a group greatly disturbed by human activities, are at increased risk of extinction. <i>Rana dybowskii</i> is an anuran species with both ecological and economic significance. Due to environmental changes and human overexploitation, it has been classified as Near-Threatened. This study integrates morphological and molecular immunological approaches to identify <i>R. dybowskii</i> populations with greater survival and disease resistance, based on 32 morphological traits and <i>MHC</i> class I and II polymorphism. Morphological results showed that compared with other populations, Yichun (YC) population had the highest fatness, the lowest IOD/HW, and the largest HW/SVL, HL/SVL, HW/HL, SL/TL. It indicates that YC population shows larger body size, wider vision and stronger jumping ability. The polymorphism of <i>MHC</i> I gene was the highest in Shangzhi (SZ) population, and the polymorphism of <i>MHC</i> II gene was the highest in YC population. Moreover, duplication, selection, and recombination occurred during evolution of <i>MHC</i> class I and II genes. Since both SZ and YC populations scored higher in this category (the variant sites, nucleotide polymorphism, amino-acid divergence/nucleotide divergence, dN/dS, Tajima’ D, etc.), they were more resistant to disease. All in all, these results indicated that YC population of the Lesser Khingan Mountains had good morphology and immune results, and <i>R. dybowskii</i> in the Lesser Khingan Mountains might be more suitable to be the original population of artificial breeding, which provided a theoretical basis for the realization of artificial breeding in the next step.</p>

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Selection of geographical populations suitable for artificial breeding of the Northeast China Brown Frog (Rana dybowskii)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年9月3日

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  • Wanli Liu,
  • Jiuchen Tao,
  • Qing Yu,
  • Tong Wu,
  • Bojian Xing,
  • Jing Bai,
  • Wenge Zhao,
  • Yufen Liu,
  • Peng Liu

摘要

Amphibians, as a group greatly disturbed by human activities, are at increased risk of extinction. Rana dybowskii is an anuran species with both ecological and economic significance. Due to environmental changes and human overexploitation, it has been classified as Near-Threatened. This study integrates morphological and molecular immunological approaches to identify R. dybowskii populations with greater survival and disease resistance, based on 32 morphological traits and MHC class I and II polymorphism. Morphological results showed that compared with other populations, Yichun (YC) population had the highest fatness, the lowest IOD/HW, and the largest HW/SVL, HL/SVL, HW/HL, SL/TL. It indicates that YC population shows larger body size, wider vision and stronger jumping ability. The polymorphism of MHC I gene was the highest in Shangzhi (SZ) population, and the polymorphism of MHC II gene was the highest in YC population. Moreover, duplication, selection, and recombination occurred during evolution of MHC class I and II genes. Since both SZ and YC populations scored higher in this category (the variant sites, nucleotide polymorphism, amino-acid divergence/nucleotide divergence, dN/dS, Tajima’ D, etc.), they were more resistant to disease. All in all, these results indicated that YC population of the Lesser Khingan Mountains had good morphology and immune results, and R. dybowskii in the Lesser Khingan Mountains might be more suitable to be the original population of artificial breeding, which provided a theoretical basis for the realization of artificial breeding in the next step.