Scholarlink

Background <p>Sepsis-associated liver injury is a major cause of morbidity and mortality, with oxidative stress, inflammation, and apoptotic signaling playing central roles in pathogenesis. Non-invasive physical modalities such as pulsed magnetic fields (PMF) and radiofrequency electromagnetic fields (RF-EMF) have shown organ-protective effects in various experimental settings; however, their combined application in hepatic inflammation has not been previously investigated.</p> Methods <p>Forty female Wistar rats were randomized into five groups: Control, LPS, LPS + PMF, LPS + RF, and LPS + PMF+RF. Acute liver injury was induced with intraperitoneal LPS. PMF and RF-EMF were applied individually or in combination. Liver tissues were analyzed by histopathology, immunohistochemistry, and RT-qPCR for oxidative (NRF2, SOD), inflammatory (TNF-α), mitochondrial apoptotic (BCL2, BAX, Cyt-C, Caspase-9), and ER stress (PERK, Caspase-12, Caspase-3) markers. Serum ALT, AST, and albumin levels were also measured.</p> Results <p>LPS significantly increased TNF-α, BAX, Cyt-C, Caspase-9, PERK, Caspase-12, and Caspase-3 expression, while decreasing NRF2, SOD, and BCL2 (all ***<i>p</i> &lt; 0.001). Both PMF and RF monotherapies partially restored these parameters; however, the combined PMF + RF application achieved the most pronounced effects at the molecular and histopathological levels, normalizing oxidative stress markers, reducing pro-inflammatory and apoptotic signaling, and improving histopathological scores (all **<i>p</i> &lt; 0.05 to ***<i>p</i> &lt; 0.001 vs. LPS). Serum AST and ALT levels were significantly reduced by PMF monotherapy, while RF and combined PMF + RF treatments also produced significant decreases compared to the LPS group, albeit to a lesser extent. Serum albumin levels remained unchanged across all groups.</p> Conclusion <p>Concurrent low-frequency PMF and RF-EMF exposure confers synergistic hepatoprotection in endotoxin-induced liver injury by modulating oxidative, inflammatory, and apoptotic pathways. These findings suggest that dual-modality PMF and RF-EMF exposure may represent a promising non-invasive experimental strategy for mitigating endotoxin-driven hepatic injury. Further studies in clinically relevant sepsis and ischemia-reperfusion models, with extended follow-up and mechanistic validation, are warranted before translational inference.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Synergistic mitigation of endotoxin-induced liver injury by low-frequency PMF and 27.12 MHz RF-EMF: a multi-biomarker experimental study

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年2月23日

查看全文
  • Bilal Turan,
  • Halil Asci,
  • Orhan Imeci,
  • Muhammet Tepebasi,
  • Arzu Ulusoy,
  • Serdar Acar,
  • Isa Karaca,
  • Orhan Akpınar,
  • Ozlem Ozmen

摘要

Background

Sepsis-associated liver injury is a major cause of morbidity and mortality, with oxidative stress, inflammation, and apoptotic signaling playing central roles in pathogenesis. Non-invasive physical modalities such as pulsed magnetic fields (PMF) and radiofrequency electromagnetic fields (RF-EMF) have shown organ-protective effects in various experimental settings; however, their combined application in hepatic inflammation has not been previously investigated.

Methods

Forty female Wistar rats were randomized into five groups: Control, LPS, LPS + PMF, LPS + RF, and LPS + PMF+RF. Acute liver injury was induced with intraperitoneal LPS. PMF and RF-EMF were applied individually or in combination. Liver tissues were analyzed by histopathology, immunohistochemistry, and RT-qPCR for oxidative (NRF2, SOD), inflammatory (TNF-α), mitochondrial apoptotic (BCL2, BAX, Cyt-C, Caspase-9), and ER stress (PERK, Caspase-12, Caspase-3) markers. Serum ALT, AST, and albumin levels were also measured.

Results

LPS significantly increased TNF-α, BAX, Cyt-C, Caspase-9, PERK, Caspase-12, and Caspase-3 expression, while decreasing NRF2, SOD, and BCL2 (all ***p < 0.001). Both PMF and RF monotherapies partially restored these parameters; however, the combined PMF + RF application achieved the most pronounced effects at the molecular and histopathological levels, normalizing oxidative stress markers, reducing pro-inflammatory and apoptotic signaling, and improving histopathological scores (all **p < 0.05 to ***p < 0.001 vs. LPS). Serum AST and ALT levels were significantly reduced by PMF monotherapy, while RF and combined PMF + RF treatments also produced significant decreases compared to the LPS group, albeit to a lesser extent. Serum albumin levels remained unchanged across all groups.

Conclusion

Concurrent low-frequency PMF and RF-EMF exposure confers synergistic hepatoprotection in endotoxin-induced liver injury by modulating oxidative, inflammatory, and apoptotic pathways. These findings suggest that dual-modality PMF and RF-EMF exposure may represent a promising non-invasive experimental strategy for mitigating endotoxin-driven hepatic injury. Further studies in clinically relevant sepsis and ischemia-reperfusion models, with extended follow-up and mechanistic validation, are warranted before translational inference.