<p>Integrative approaches combining bioinformatics tools, and genomic data have enabled the characterization of satellite DNA (satDNA), providing an understanding of the composition and the evolutionary processes that have shaped the genomes of different organisms. In this study, we performed a comparative genomic analysis between leaf-cutting ant <i>Acromyrmex octospinosus</i> (Reich, 1793) and its social parasite <i>Acromyrmex insinuator</i> Schultz et al. 1998. We characterized, in terms of diversity, organization pattern and sequence divergence, satDNA families from the genome of these species and provided the first insights into the evolution of these repetitive sequences in the leaf-cutting ants. Nine satDNA families were recovered and shared in the genomes of both <i>Acromyrmex</i> Mayr, 1865 species, which supports previous molecular data suggesting a recent evolutionary origin of the parasite directly from the host species. Evolutionary landscapes (abundance versus divergence) suggest the occurrence of amplification and contraction events, until the almost complete degeneration of satDNA families between two species from a common ancestral library. This study represents the first step toward understanding the organization and evolution of satellite DNA in the genomes of leaf-cutting ants.</p>