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<p>Global warming can interact with eutrophication at regional and local scales, promoting changes in the composition of microbial communities and having distinct effects on trophic levels of freshwater ecosystems. The use of experimental strategies associated with metabarcoding techniques can help elucidate the responses of prokaryotic and eukaryotic microbiota to the individual and combined effects of these stressors. In this study, we experimentally evaluated the effects of eutrophication and water warming on planktonic microbiota in terms of taxonomic composition and trophic levels (i.e., autotrophic and heterotrophic). The study was conducted in 30 mesocosms, randomly distributed in two nutrient concentrations (oligotrophic and eutrophic) and three temperature conditions based on future scenarios of global warming. Water and planktonic microbiota were obtained from an oligotrophic lake and monitored over 30&#xa0;days of exposure to include combinations of temperature and nutrients. We found a strong effect of eutrophication on the composition of prokaryotic and eukaryotic microbiota. However, no isolated effects of temperature were detected for the communities. We found no effects of these two stressors on beta diversity; in other words, community dispersion remained the same across treatments. These results underscore the predominant role of eutrophication in restructuring aquatic microbial communities as assessed by DNA metabarcoding. While warming did not have strong independent effects, its interaction with eutrophication can alter the structure and composition at different trophic levels of communities.</p>

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Effects of eutrophication and rising water temperature on prokaryotic and eukaryotic microbiota assessed by metabarcoding in a tropical freshwater mesocosm experiment

  • 期刊论文

  • 发表日期：

    2025年12月12日

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  • João Carlos Nabout,
  • Beatriz Barbosa Araújo Martins,
  • Ariany Tavares de Andrade,
  • Marcela Fernandes de Almeida,
  • Cíntia Pelegrineti Targueta,
  • Jocilaine Santos de Jesus,
  • Karine Borges Machado,
  • Thannya Nascimento Soares

摘要

Global warming can interact with eutrophication at regional and local scales, promoting changes in the composition of microbial communities and having distinct effects on trophic levels of freshwater ecosystems. The use of experimental strategies associated with metabarcoding techniques can help elucidate the responses of prokaryotic and eukaryotic microbiota to the individual and combined effects of these stressors. In this study, we experimentally evaluated the effects of eutrophication and water warming on planktonic microbiota in terms of taxonomic composition and trophic levels (i.e., autotrophic and heterotrophic). The study was conducted in 30 mesocosms, randomly distributed in two nutrient concentrations (oligotrophic and eutrophic) and three temperature conditions based on future scenarios of global warming. Water and planktonic microbiota were obtained from an oligotrophic lake and monitored over 30 days of exposure to include combinations of temperature and nutrients. We found a strong effect of eutrophication on the composition of prokaryotic and eukaryotic microbiota. However, no isolated effects of temperature were detected for the communities. We found no effects of these two stressors on beta diversity; in other words, community dispersion remained the same across treatments. These results underscore the predominant role of eutrophication in restructuring aquatic microbial communities as assessed by DNA metabarcoding. While warming did not have strong independent effects, its interaction with eutrophication can alter the structure and composition at different trophic levels of communities.