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Background <p>In a ligature-induced periodontitis rat model, we tested oral citral (100 mg/kg, gavage) for 6 or 14 days and quantified alveolar bone loss, gingival cytokines, nitric oxide (NO), reactive oxygen species (ROS), MMP-2 activity, and plasma TNF-α.</p> Methods <p>Adult male rats received a ligature around the first lower right molar for 7 or 15 days to induce periodontitis, while control animals remained unligated. Alveolar bone loss was assessed by measuring the cementoenamel junction–bone crest distance. Gingival cytokines (TNF-α, IL-1β, IL-6, IL-10) and plasma TNF-α were determined by ELISA, gingival NO by chemiluminescence, MMP-2 activity by gelatin zymography, and local ROS by in situ detection.</p> Results <p>Citral did not affect healthy gingiva. In ligature-induced periodontitis, a 14-day citral treatment significantly reduced alveolar bone loss, downregulated pro-inflammatory cytokines, increased IL-10, decreased plasma TNF-α levels, and inhibited both NO production and MMP-2 activity. ROS accumulation in the gingival tissue was also attenuated.</p> Conclusion <p>These findings suggest that oral citral exhibits anti-inflammatory and antioxidant effects, thereby limiting alveolar bone loss in experimental periodontitis.</p>

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Oral citral attenuates alveolar bone loss and inflammation in ligature-induced periodontitis in rats

  • 期刊论文

  • 发表日期：

    2025年10月27日

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  • Wanderson S. Santos,
  • Isabelly G. Solon,
  • Pedro H. Lopes,
  • Glauce C. Nascimento,
  • Luiz G. S. Branco

摘要

Background

In a ligature-induced periodontitis rat model, we tested oral citral (100 mg/kg, gavage) for 6 or 14 days and quantified alveolar bone loss, gingival cytokines, nitric oxide (NO), reactive oxygen species (ROS), MMP-2 activity, and plasma TNF-α.

Methods

Adult male rats received a ligature around the first lower right molar for 7 or 15 days to induce periodontitis, while control animals remained unligated. Alveolar bone loss was assessed by measuring the cementoenamel junction–bone crest distance. Gingival cytokines (TNF-α, IL-1β, IL-6, IL-10) and plasma TNF-α were determined by ELISA, gingival NO by chemiluminescence, MMP-2 activity by gelatin zymography, and local ROS by in situ detection.

Results

Citral did not affect healthy gingiva. In ligature-induced periodontitis, a 14-day citral treatment significantly reduced alveolar bone loss, downregulated pro-inflammatory cytokines, increased IL-10, decreased plasma TNF-α levels, and inhibited both NO production and MMP-2 activity. ROS accumulation in the gingival tissue was also attenuated.

Conclusion

These findings suggest that oral citral exhibits anti-inflammatory and antioxidant effects, thereby limiting alveolar bone loss in experimental periodontitis.