We demonstrate that the observed baryon asymmetry in the Universe can be accommodated in the extended Standard Model with sequential fourth generation fermions (SM4). We first construct the dimension-6 effective operators of the type \( -i\left({\Phi}^{\dagger}\Phi \right){\overline{F}}_L\Phi {f}_R \) induced by fourth generation quarks, which carry the CP violation (CPV) source from the 4 × 4 Cabibbo-Kobayashi-Maskawa (CKM) matrix, Φ (FL, fR) being a Higgs double (left-handed fermion doublet, right-handed fermion singlet). The required inputs of the fourth generation fermion masses were derived in our previous dispersive analyses on heavy quark decays and neutral meson mixing. The similar framework allows the determination of the 4 × 4 CKM matrix elements \( {V}_{i{b}^{\prime }} \) , i = u, c and t, such that the strength of the CPV source can be evaluated unambiguously. The dimension-6 operators associated with fourth generation leptons, as implemented into the formalism for the electroweak baryogenesis in the literature, lead to the baryon-over-entropy ratio ηB ≈ 10−10.